RESUMO
Soil microorganisms with diverse bioactive compounds such as Streptomyces are appreciated as valuable resources for the discovery of eco-friendly fungicides. This study isolated a novel Streptomyces from soil samples collected in the organic green tea fields in South Korea. The isolation process involved antifungal activity screening around 2400 culture extracts, revealing a strain designated as S. collinus Inha504 with remarkable antifungal activity against diverse phytopathogenic fungi. S. collinus Inha504 not only inhibited seven phytopathogenic fungi including Fusarium oxysporum and Aspergillus niger in bioassays and but also showed a control effect against F. oxysporum infected red pepper, strawberry, and tomato in the in vivo pot test. Genome mining of S. collinus Inha504 revealed the presence of the biosynthetic gene cluster (BGC) in the chromosome encoding a polyene macrolide which is highly homologous to the lucensomycin (LCM), a compound known for effective in crop disease control. Through genetic confirmation and bioassays, the antifungal activity of S. collinus Inha504 was attributed to the presence of LCM BGC in the chromosome. These results could serve as an effective strategy to select novel Streptomyces strains with valuable biological activity through bioassay-based screening and identify biosynthetic gene clusters responsible for the metabolites using genome mining approach.
Assuntos
Antifúngicos , Streptomyces , Antifúngicos/metabolismo , Lucensomycin/metabolismo , Streptomyces/genética , Streptomyces/metabolismo , Fungos/genética , Família Multigênica , SoloRESUMO
Actinobacteria are among the most prolific sources of medically and agriculturally important compounds, derived from their biosynthetic gene clusters (BGCs) for specialized (secondary) pathways of metabolism. Genomics witnesses that the majority of actinobacterial BGCs are silent, most likely due to their low or zero transcription. Much effort is put into the search for approaches towards activation of silent BGCs, as this is believed to revitalize the discovery of novel natural products. We hypothesized that the global transcriptional factor AdpA, due to its highly degenerate operator sequence, could be used to upregulate the expression of silent BGCs. Using Streptomyces cyanogenus S136 as a test case, we showed that plasmids expressing either full-length adpA or its DNA-binding domain led to significant changes in the metabolome. These were evident as changes in the accumulation of colored compounds, bioactivity, as well as the emergence of a new pattern of secondary metabolites as revealed by HPLC-ESI-mass spectrometry. We further focused on the most abundant secondary metabolite and identified it as the polyene antibiotic lucensomycin. Finally, we uncovered the entire gene cluster for lucensomycin biosynthesis (lcm), that remained elusive for five decades until now, and outlined an evidence-based scenario for its adpA-mediated activation.
Assuntos
Antibacterianos/farmacologia , Vias Biossintéticas/efeitos dos fármacos , Regulação Bacteriana da Expressão Gênica/genética , Lucensomycin/metabolismo , Proteínas de Bactérias/metabolismo , Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Genes Bacterianos/efeitos dos fármacos , Genes Reguladores/efeitos dos fármacos , Metabolismo Secundário/genética , Streptomyces/efeitos dos fármacos , Fatores de Transcrição/efeitos dos fármacos , Fatores de Transcrição/metabolismoRESUMO
We report the identification, cloning, heterologous expression and functional characterization of a novel antifungal peptide named lucimycin from the common green bottle fly Lucilia sericata. The lucimycin cDNA was isolated from a library of genes induced during the innate immune response in L. sericata larvae, which are used as therapeutic maggots. The peptide comprises 77 amino acid residues with a molecular mass of 8.2 kDa and a pI of 6.6. It is predicted to contain a zinc-binding motif and to form a random coil, lacking ß-sheets or other secondary structures. Lucimycin was active against fungi from the phyla Ascomycota, Basidiomycota and Zygomycota, in addition to the oomycete Phytophtora parasitica, but it was inactive against bacteria. A mutant version of lucimycin, lacking the four C-terminal amino acid residues, displayed 40-fold lower activity. The activity of lucimycin against a number of highly-destructive plant pathogens could be exploited to produce transgenic crops that are resistant against fungal diseases.
Assuntos
Antifúngicos/metabolismo , Lucensomycin/metabolismo , Peptídeos/metabolismo , Animais , Larva , Análise de Sequência de ProteínaRESUMO
The binding of lucensomycin to unilamellar phospholipid/cholesterol vesicles and to colloidal emulsions of cholesterol in aqueous solutions was studied by monitoring the changes in the electronic absorption spectra of the polyene antibiotic. The total extent of the absorption variations was a direct function of cholesterol concentration and quite independent of the nature of the emulsion. The rate of binding, relatively slow in the colloidal systems, was greatly enhanced when cholesterol was included in phospholipid-containing membranes. The rate of lucensomycin binding to colloidal cholesterol increased with increasing cholesterol concentrations and/or stirring the heterogeneous suspension. The time course of lucensomycin binding to vesicles appeared to be independent of the concentrations of phospholipids and cholesterol.
Assuntos
Antifúngicos/metabolismo , Lucensomycin/metabolismo , Membranas Artificiais , Fosfolipídeos/farmacologia , Ligação Competitiva , Colesterol , Emulsões , Espectrofotometria UltravioletaRESUMO
The binding of the polyenic antibiotic lucensomycin to native or modified human erythrocyte ghosts and to model membranes has been studied by monitoring the absorbance variations of the polyene at 320 nm. The non-steroidal components of the membranes (such as proteins and phospholipids) seem to affect the rate of the individual reaction steps leading to the formation of cholesterol-lucensomycin complexes rather than the ratio among these heterologous aggregates at equilibrium.
Assuntos
Antifúngicos/metabolismo , Membrana Eritrocítica/metabolismo , Lucensomycin/metabolismo , Membranas Artificiais , Sítios de Ligação , Colesterol , Humanos , Cinética , Matemática , Lipídeos de Membrana/metabolismo , Fosfatidilcolinas , EspectrofotometriaRESUMO
The variations of optical density and fluorescence of lucensomycin are good indices of the binding of this polyenic antibiotic to membranes. The former parameter reflects more generally the binding to any site present in the membrane, while the latter is more specific for binding to cholesterol. Equilibrium titration experiments performed in the presence of an excess of membrane-bound cholesterol suggest that lucensomycin self-associates and that the binding and polymerization sites of the antibiotic are identical or quasi-identical; hence polymerization leads to a loss of binding sites and vice versa. The non-steroidal components of the membrane (such as proteins and lipids) seem to affect the rate of the individual reaction leading to the formation of the cholesterol-lucensomycin complexes, rather than the ratio among these heterologous aggregates at equilibrium. Polyene concentrations, which induce detectable proton release from unilamellar vesicles, are at least two orders of magnitude higher than those necessary to perform a spectroscopic titration of membrane cholesterol.
Assuntos
Antifúngicos/metabolismo , Colesterol/metabolismo , Lucensomycin/metabolismo , Lipídeos de Membrana/metabolismo , Permeabilidade da Membrana Celular , Membrana Eritrocítica/metabolismo , Fluorescência , Humanos , Cinética , Espectroscopia de Ressonância Magnética , Matemática , Polímeros/metabolismo , Relação Estrutura-AtividadeRESUMO
The effect of the polyene antibiotic etruscomycin on the permeability of large unilamellar lipid vesicles was investigated. Proton leakage was induced in egg-yolk phosphatidylcholine (EPC) vesicles only when sterol was present in the membrane; the extent of leakage was limited. High etruscomycin/lipid ratios (R) were necessary (R greater than 0.1). Higher percentages of sterol increased the permeability, slightly more strongly for ergosterol than for cholesterol. Dipalmitoylphosphatidylcholine (DPPC) vesicles were more sensitive to permeability inducement, even in the absence of sterol in the bilayer (inducement for R greater than 0.06). The interactions of etruscomycin with the vesicles were examined by circular dichroism, fluorescence and 31P-NMR. In the range of antibiotic concentration where permeability was induced, R greater than 0.1 for EPC vesicles, R greater than 0.06 for DPPC vesicles, etruscomycin exhibited characteristic circular dichroism spectra independent of the presence of sterol. Under the same conditions, 31P-NMR and fluorescence studies indicated a destruction or a fusion of the vesicle bilayer. At lower etruscomycin concentrations (R less than 0.03), the etruscomycin circular dichroism spectra were different, indicating that the interaction with membranes containing ergosterol differed from that with membranes containing cholesterol. From correlating the increase in fluorescence intensity with this interaction, as well as from exchange experiments, it was inferred that etruscomycin at a low antibiotic/lipid ratio is more strongly bound to ergosterol-containing vesicles than to cholesterol-containing vesicles. These results and their comparison with the results obtained with other polyene antibiotics indicate that at low R etruscomycin resembles amphotericin rather than filipin in its preferential binding to ergosterol-containing vesicles. At higher R, that is in conditions where permeability is induced, the selectivity is different. The corresponding mechanism seems not to involve the formation of an etruscomycin-sterol channel, since the hydrophobic chain of the complex would be too short to form a channel.
Assuntos
Antifúngicos/farmacologia , Permeabilidade da Membrana Celular/efeitos dos fármacos , Lipossomos/metabolismo , Lucensomycin/farmacologia , Prótons , Colesterol/metabolismo , Dicroísmo Circular , Ergosterol/metabolismo , Concentração de Íons de Hidrogênio , Cinética , Lucensomycin/metabolismo , Espectroscopia de Ressonância Magnética , Fosfatidilcolinas/metabolismo , Surfactantes Pulmonares/metabolismo , Espectrometria de FluorescênciaRESUMO
Two methods, biological and spectroscopic, were used to determine the avidity of the polyene antibiotic Etruscomycin for cholesterol and ergosterol. The biological method consisted of measuring the inhibitory potency of both sterols on the Etruscomycin-induced damage to erythrocytes and fungi. The spectroscopic method consisted of recording of series of differential spectra in a number of solvents of different composition. The results obtained showed that cholesterol protected erythrocytes and candida albicans against the damaging action of Etruscomycin more efficiently than ergosterol did and that Etruscomycin-cholesterol complexes were more resistant to interruption by organic solvents than Etruscomycin-ergosterol complexes. These results and their comparison with the results obtained with other polyene antibiotics indicate that Etruscomycin resembles filipin in that it binds more avidly to cholesterol than to ergosterol. This implies that the length of the hydrophobic chain rather than the presence of the amino sugar determines sterol preference. The spectral method that we used can have general application for the quantitative measurement of complex formation between polyenes and sterols.